Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.
Identifieur interne : 001118 ( Main/Exploration ); précédent : 001117; suivant : 001119Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.
Auteurs : Janne Tynell [Finlande] ; Veera Westenius [Finlande] ; Esa Rönkkö [Finlande] ; Vincent J. Munster [États-Unis] ; Krister Melén [Finlande] ; Pamela Österlund [Finlande] ; Ilkka Julkunen [Finlande]Source :
- The Journal of general virology [ 1465-2099 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Adulte, Cellules dendritiques (immunologie), Cellules dendritiques (virologie), Chimiokine CXCL10 (métabolisme), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Humains, Immunité innée, Macrophages (immunologie), Macrophages (virologie), Protéines de résistance aux myxovirus (métabolisme), Protéines virales (analyse), Réplication virale, Virus du SRAS (immunologie), Virus du SRAS (physiologie).
- MESH :
- analyse : ARN viral, Protéines virales.
- immunologie : Cellules dendritiques, Coronavirus du syndrome respiratoire du Moyen-Orient, Macrophages, Virus du SRAS.
- métabolisme : Chimiokine CXCL10, Protéines de résistance aux myxovirus.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Virus du SRAS.
- virologie : Cellules dendritiques, Macrophages.
- Adulte, Humains, Immunité innée, Réplication virale.
English descriptors
- KwdEn :
- Adult, Chemokine CXCL10 (metabolism), Dendritic Cells (immunology), Dendritic Cells (virology), Humans, Immunity, Innate, Macrophages (immunology), Macrophages (virology), Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (physiology), Myxovirus Resistance Proteins (metabolism), RNA, Viral (analysis), SARS Virus (immunology), SARS Virus (physiology), Viral Proteins (analysis), Virus Replication.
- MESH :
- chemical , analysis : RNA, Viral, Viral Proteins.
- chemical , metabolism : Chemokine CXCL10, Myxovirus Resistance Proteins.
- immunology : Dendritic Cells, Macrophages, Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- physiology : Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- virology : Dendritic Cells, Macrophages.
- Adult, Humans, Immunity, Innate, Virus Replication.
Abstract
In this study we assessed the ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to replicate and induce innate immunity in human monocyte-derived macrophages and dendritic cells (MDDCs), and compared it with severe acute respiratory syndrome coronavirus (SARS-CoV). Assessments of viral protein and RNA levels in infected cells showed that both viruses were impaired in their ability to replicate in these cells. Some induction of IFN-λ1, CXCL10 and MxA mRNAs in both macrophages and MDDCs was seen in response to MERS-CoV infection, but almost no such induction was observed in response to SARS-CoV infection. ELISA and Western blot assays showed clear production of CXCL10 and MxA in MERS-CoV-infected macrophages and MDDCs. Our data suggest that SARS-CoV and MERS-CoV replicate poorly in human macrophages and MDDCs, but MERS-CoV is nonetheless capable of inducing a readily detectable host innate immune response. Our results highlight a clear difference between the viruses in activating host innate immune responses in macrophages and MDDCs, which may contribute to the pathogenesis of infection.
DOI: 10.1099/jgv.0.000351
PubMed: 26602089
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001374
- to stream PubMed, to step Curation: 001374
- to stream PubMed, to step Checkpoint: 000F84
- to stream Ncbi, to step Merge: 001368
- to stream Ncbi, to step Curation: 001368
- to stream Ncbi, to step Checkpoint: 001368
- to stream Main, to step Merge: 001122
- to stream Main, to step Curation: 001118
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.</title><author><name sortKey="Tynell, Janne" sort="Tynell, Janne" uniqKey="Tynell J" first="Janne" last="Tynell">Janne Tynell</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Westenius, Veera" sort="Westenius, Veera" uniqKey="Westenius V" first="Veera" last="Westenius">Veera Westenius</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Ronkko, Esa" sort="Ronkko, Esa" uniqKey="Ronkko E" first="Esa" last="Rönkkö">Esa Rönkkö</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J" last="Munster">Vincent J. Munster</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Melen, Krister" sort="Melen, Krister" uniqKey="Melen K" first="Krister" last="Melén">Krister Melén</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Osterlund, Pamela" sort="Osterlund, Pamela" uniqKey="Osterlund P" first="Pamela" last="Österlund">Pamela Österlund</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Julkunen, Ilkka" sort="Julkunen, Ilkka" uniqKey="Julkunen I" first="Ilkka" last="Julkunen">Ilkka Julkunen</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26602089</idno><idno type="pmid">26602089</idno><idno type="doi">10.1099/jgv.0.000351</idno><idno type="wicri:Area/PubMed/Corpus">001374</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001374</idno><idno type="wicri:Area/PubMed/Curation">001374</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001374</idno><idno type="wicri:Area/PubMed/Checkpoint">000F84</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000F84</idno><idno type="wicri:Area/Ncbi/Merge">001368</idno><idno type="wicri:Area/Ncbi/Curation">001368</idno><idno type="wicri:Area/Ncbi/Checkpoint">001368</idno><idno type="wicri:Area/Main/Merge">001122</idno><idno type="wicri:Area/Main/Curation">001118</idno><idno type="wicri:Area/Main/Exploration">001118</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.</title><author><name sortKey="Tynell, Janne" sort="Tynell, Janne" uniqKey="Tynell J" first="Janne" last="Tynell">Janne Tynell</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Westenius, Veera" sort="Westenius, Veera" uniqKey="Westenius V" first="Veera" last="Westenius">Veera Westenius</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Ronkko, Esa" sort="Ronkko, Esa" uniqKey="Ronkko E" first="Esa" last="Rönkkö">Esa Rönkkö</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J" last="Munster">Vincent J. Munster</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Melen, Krister" sort="Melen, Krister" uniqKey="Melen K" first="Krister" last="Melén">Krister Melén</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Osterlund, Pamela" sort="Osterlund, Pamela" uniqKey="Osterlund P" first="Pamela" last="Österlund">Pamela Österlund</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Julkunen, Ilkka" sort="Julkunen, Ilkka" uniqKey="Julkunen I" first="Ilkka" last="Julkunen">Ilkka Julkunen</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Health and Welfare (THL), Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>National Institute for Health and Welfare (THL), Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Chemokine CXCL10 (metabolism)</term><term>Dendritic Cells (immunology)</term><term>Dendritic Cells (virology)</term><term>Humans</term><term>Immunity, Innate</term><term>Macrophages (immunology)</term><term>Macrophages (virology)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (physiology)</term><term>Myxovirus Resistance Proteins (metabolism)</term><term>RNA, Viral (analysis)</term><term>SARS Virus (immunology)</term><term>SARS Virus (physiology)</term><term>Viral Proteins (analysis)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Adulte</term><term>Cellules dendritiques (immunologie)</term><term>Cellules dendritiques (virologie)</term><term>Chimiokine CXCL10 (métabolisme)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term><term>Humains</term><term>Immunité innée</term><term>Macrophages (immunologie)</term><term>Macrophages (virologie)</term><term>Protéines de résistance aux myxovirus (métabolisme)</term><term>Protéines virales (analyse)</term><term>Réplication virale</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chemokine CXCL10</term><term>Myxovirus Resistance Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules dendritiques</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Macrophages</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Dendritic Cells</term><term>Macrophages</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chimiokine CXCL10</term><term>Protéines de résistance aux myxovirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules dendritiques</term><term>Macrophages</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Dendritic Cells</term><term>Macrophages</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Humans</term><term>Immunity, Innate</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Humains</term><term>Immunité innée</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study we assessed the ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to replicate and induce innate immunity in human monocyte-derived macrophages and dendritic cells (MDDCs), and compared it with severe acute respiratory syndrome coronavirus (SARS-CoV). Assessments of viral protein and RNA levels in infected cells showed that both viruses were impaired in their ability to replicate in these cells. Some induction of IFN-λ1, CXCL10 and MxA mRNAs in both macrophages and MDDCs was seen in response to MERS-CoV infection, but almost no such induction was observed in response to SARS-CoV infection. ELISA and Western blot assays showed clear production of CXCL10 and MxA in MERS-CoV-infected macrophages and MDDCs. Our data suggest that SARS-CoV and MERS-CoV replicate poorly in human macrophages and MDDCs, but MERS-CoV is nonetheless capable of inducing a readily detectable host innate immune response. Our results highlight a clear difference between the viruses in activating host innate immune responses in macrophages and MDDCs, which may contribute to the pathogenesis of infection. </div></front></TEI><affiliations><list><country><li>Finlande</li><li>États-Unis</li></country><region><li>Montana</li></region></list><tree><country name="Finlande"><noRegion><name sortKey="Tynell, Janne" sort="Tynell, Janne" uniqKey="Tynell J" first="Janne" last="Tynell">Janne Tynell</name></noRegion><name sortKey="Julkunen, Ilkka" sort="Julkunen, Ilkka" uniqKey="Julkunen I" first="Ilkka" last="Julkunen">Ilkka Julkunen</name><name sortKey="Melen, Krister" sort="Melen, Krister" uniqKey="Melen K" first="Krister" last="Melén">Krister Melén</name><name sortKey="Osterlund, Pamela" sort="Osterlund, Pamela" uniqKey="Osterlund P" first="Pamela" last="Österlund">Pamela Österlund</name><name sortKey="Ronkko, Esa" sort="Ronkko, Esa" uniqKey="Ronkko E" first="Esa" last="Rönkkö">Esa Rönkkö</name><name sortKey="Westenius, Veera" sort="Westenius, Veera" uniqKey="Westenius V" first="Veera" last="Westenius">Veera Westenius</name></country><country name="États-Unis"><region name="Montana"><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J" last="Munster">Vincent J. Munster</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001118 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001118 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26602089
|texte= Middle East respiratory syndrome coronavirus shows poor replication but significant induction of antiviral responses in human monocyte-derived macrophages and dendritic cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26602089" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |